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Fig. 6
ERK inhibition does not affect initial artery differentiation. (A,E) Quantification of marker expression following treatment with the indicated compounds from 10 to 24 hpf (A) or 24 to 28 hpf (E). Numbers of embryos used for analysis and probes are indicated. *P<0.05, ***P<0.001; ns, not statistically significant. (B-D) Whole-mount in situ hybridization on 24 hpf embryos treated as indicated from 10 hpf using probes against efnb2a (B), hey2 (C) or notch1b (D). Arrows denote staining in the DA.
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