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Fig. 4
Z tie-1 and Z tie-2 expression in tail endothelium. Whole mount in situ hybridization of embryos at 24HPFhybridized with probes forZ flk-1 (A,B), Z tie-1 (C,D), and Z tie-2 (E,F). B,D,F: Higher magnification views of the corresponding boxes on A,C,E, respectively. Dorsal aorta, arrows; cardinal vein, arrowheads; intersegmental vessels, open arrows. Note a marked absence of intersegmental vessel staining in Z tie-2 embryos. Original magnification: A,C,E, x 60; B,D,F, x 300.
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