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Fig. 6
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Fig. 6
In vivo action of Ptger4 antagonist (GW627368X) on hCG induced oocyte maturation and ovulation.
(a) Relative expression of ptger genes in FG stage follicles. (b) Morphology of follicles from npr+/+ and npr-/- fish treated with hCG(10 IU/µl) or hCG + GW627368X(10 µM) for 4 hours. (c) Oocyte maturation and ovulation rates in npr+/+ and npr-/- fish treated with hCG or hCG + GW627368X for 4 hours. Each value represents the mean value ± SEM of triplicate assays from 3 independent experiments. (***P < 0.001 vs control).
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