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Fig. 3
Dominant repressor Hoxb8a down-regulates the expression of cxcr4b and cxcr7b. (A) Fluorescent images of cldnb:lyngfp in cldnb4.2:gal4 x UAS:Hoxb8aEnRHA embryos and control siblings at 45 hpf. Red arrowheads indicate the position of the primordium. (B) Quantification of the migration at 45 hpf (control, n = 56; cldnb4.2:gal4 x UAS:Hoxb8aEnRHA, n = 62). Expression of (C) cxcr4b, (D) cxcr7b, and (E) lef1 in cldnb4.2:gal4 x UAS:Hoxb8aEnRHA and control siblings at 30 hpf. ISH was followed by HA immunostaining (brown). (Scale bar: 25 µm.)
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