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Fig. 3
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Fig. 3
wwtr1-MO injection effect on the expression of thyroid transcription factors and thyroid specific genes. 48 hpf zebrafish embryos uninjected (uninj) (A and C) and injected with standard control-MO (scMO) (E, G, I) and wwtr1-MO (B, D, F, H, J), hybridized with probes for thyroid transcription factors nkx2.1a, pax2a and pax8, and thyroid differentiation genes tg and slc5a5 (A–H). Arrowheads indicate the thyroid gland. Asterisk depicts specific nkx2.1a mRNA signal over high staining background (B). Scale bar = 200 µm. Anterior is to the left in lateral view.
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Reprinted from Mechanisms of Development, 138 Pt 3, Pappalardo, A., Porreca, I., Caputi, L., De Felice, E., Schulte-Merker, S., Zannini, M., Sordino, P., Thyroid development in zebrafish lacking Taz, 268-78, Copyright (2015) with permission from Elsevier. Full text @ Mech. Dev.