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Fig. 1
Characterization of the log Mutant Phenotype
(A–I) Expression of cardiac markers in (WT) and log mutant (MUT) embryos. While at 1.0 dpf the linear heart tube (black arrowhead) was apparent in all embryos (“WT/MUT”), at 2.25 dpf log mutant hearts were unlooped, with the ventricle directly above the atrium (yellow and red arrowheads, respectively).
(J–M) At 4.0 dpf, semicircular canals in the otic vesicles (black arrow heads in J and K) and elongation of the pectoral fins (black arrowheads in L and M) were absent in mutant embryos.
(N) Change in head/trunk angle over time in WT and mutant embryos (10 WT and 20 mutants measured per time point).
See also Figure S1.