ZFIN Image: Windner et al., 2015, Fig. 4

Image

Figure Caption

Fig. 4

Ripply1 terminates tbx6 and mesp-b expression in the pre-somitic mesoderm. (A-D) Control and Ripply1 MO-injected embryos at the 12-somite stage labeled for tbx6 (A, mRNA; B, protein), and mesp-ba (C) and mesp-bb mRNA (D). All markers are upregulated in the absence of Ripply1. MF20 (B, red) labels slow muscle progenitors. (E-G) Tg(hsp70l:ripply1^myc) embryos and non-transgenic sibling 0.5h (E) and 1h (F,G) post heat shock. Transgenics lose expression of Tbx6 protein (E) and mesp-ba mRNA (G), tbx6 mRNA is only slightly reduced (F). (H-J) Tg(hsp70l:mesp-ba^myc) embryos and non-transgenic siblings 1h post heat shock. Ubiquitous expression of Mesp-ba leads to upregulation of ripply1 mRNA in wild type and tbx6 mutants (H,I). mesp-ba mRNA is not ubiquitously upregulated after Tg(hsp70l:mesp-ba^myc): levels are low in the paraxial mesoderm, endogenous mesp-ba expression (arrowheads) is downregulated (J). (K) Control and Mesp-b MO-injected embryos labeled for mesp-ba mRNA. Scale bars: 100µm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development