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Fig. 4
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Fig. 4
Overexpression or knockdown of dscr5 has no effect on mesoderm and neural patterning. Embryos were injected as indicated and in situ hybridization was performed using the indicated markers. (A-I) Animal pole view shows the expression pattern of chordin (A-C), eve1 (D-F) and ntl (G-I) at 50% epiboly. (J-R) Dorsoanterior view (J-O) and dorsal view (P-R), with anterior to the top, show the expression pattern of nk2.1b (J-L), nk2.2 (M-O) and patched 1 (ptc1) (P-R) at 12.5 hpf. Note the broader neuroectoderm stained by ptc1 in dscr5 RNA- and dscr5MO-injected embryos.
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