Fig. 3 unc45b mRNA expression correlates to non-muscle myosin but not muscle myosin in paraxial mesoderm. In situ hybridization, high magnification view of developing somites in zebrafish embryos at the 2-somite, (A, D, G, J), 5-somite (B, E, H, K) and 10-somite (C, F, I, L) stages. Non-muscle myosin (myh10, A–C) and unc45b (D–F) were expressed consistently in both adaxial cells (arrows) and lateral paraxial mesoderm (arrowheads) at all stages. In contrast, slow-muscle myosin (smyhc1, G–I) was expressed only in adaxial cells (arrows) at these stages, beginning lateral migration into the somites only at the latest stages examined (white arrowhead in I). Fast-muscle specific myosin (myhc4, J–L) was expressed after the 5-somite stage, and only in adaxial cells (arrows).
Reprinted from Developmental Biology, 390, Myhre, J.L., Hills, J.A., Jean, F., Pilgrim, D.B., Unc45b is essential for early myofibrillogenesis and costamere formation in zebrafish, 26-40, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.