Fig. 3

Fig. 3 The smu mutant phenotype is rescued by injection of smoothened RNA. (A,B) At 24 h, smu^b641 embryos (B) had almost no nkx2.2 expression compared with wild type (A). (C) Injection of 50 pg smoothened RNA at the two-cell stage partially rescued nkx2.2 expression. (D,E) At 18 h, smu^b641 embryos (E) lacked islet1-expressing primary motoneurons in posterior trunk and tail compared with wild type (D). (F) Injection of 100 pg smoothened RNA at the one-cell stage completely rescued primary motoneuron phenotype in smu^b641 mutants. (G,H) At 24 h, smu^b641 embryos (H) had almost no patched1 expression compared with wild type (G). (I) Injection of 50 pg smoothened RNA at the two-cell stage almost completely rescued patched1 expression. Side views, dorsal towards the top, anterior towards the left. Scale bar: 75 μm in A-C; 50 μm in D-F; 62 μm in G-I.