ZFIN Image: Fang et al., 2013, Fig. 2

Image

Figure Caption

Fig. 2

Induction of Jak1/Stat3 pathway members and ligands after cardiac injury. (A and B) In situ hybridization revealing Jak1/Stat3 pathway members (shown here are il6st and socs3b) induced in an organ-wide manner at 1 dpa (A), and then restricted to the injury site at 7 dpa (B). Dashed lines indicate approximate amputation plane. Brackets indicate injury site. (C) qPCR from ventricular RNA samples revealed induction of il11a, il11b, and lif at 1 and 7 dpa. Data are mean ± SEM n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, Student t test (unpaired, two-tailed). Expression levels were normalized to that of β-actin2, and further normalized to that of the uninjured sample. (D) il11a expression was induced in an organ-wide manner in endocardial cells at 1 dpa, and localized to the injury site at 7 dpa. Dashed line indicates approximate amputation plane. Brackets indicate injury site. (E) RT-PCR of samples from purified endocardial/endothelial (fli1:EGFP⁺) cells or pu.1-enriched cells at 1 dpa. il11a was detected in fli1:EGFP⁺ samples, similar to the Vegf receptor flk1. lif was detected in pu.1-enriched samples; it was undetectable when the same amount of total ventricular RNA was used for amplification (total). il11b was detected in pu.1-enriched samples and less so in fli1:EGFP⁺ samples. anf and tcf21 are markers for cardiomyocytes and epicardium, respectively, and were detected in total ventricular RNA samples. (Scale bars, 50 μm.)

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA