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Fig. 6
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Fig. 6 Vip- and Uts1-positive neurons develop within different MA domains. (A-H′′) Two-color FISH of 3-dpf (A-D′′) and 2-dpf (E-H′′) wild-type zebrafish embryos to detect the relative expression of vip, uts1, otpb, sim1a, fezf2 and foxb1.2 in the MA. uts1 is co-expressed with foxb1.2 (A-A′′), sim1a (B-B′′) and fezf2 (C-C′′) but not with otpb (D-D′′). By contrast, vip is not co-expressed with foxb1.2 (E-E′′) and fezf2 (G-G′′), whereas it is co-expressed with sim1a (F-F′′) and otpb (H-H′′). Maximum projections of 10-15 μm confocal stacks in the dorsal view are shown. Scale bar: 60 μm.
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