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Fig. 2 Asymmetric gene expression is disturbed in lpar3 and atx morphants. (A-O) WISH of asymmetry genes spaw (A-E), pitx2 (F-J) and lefty1 and lefty2 (K-O) in zebrafish embryos injected with designated MOs are shown. Open arrowheads, lateral plate mesoderm; arrow, diencephalon; solid arrowheads, heart precursor. (P-R) Asymmetry genes spaw (P), pitx2 (Q) and leftys (R) were quantified in MO-injected embryos. For each treatment, embryos from two independent batches were analyzed. Gene expression in diencephalon (labeled B) and heart precursor (labeled H) were analyzed separately in R.
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