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Fig. 5
Osr, wnt2ba and pax2a genes are co-expressed in the kidney anlage. (A-C) Dorsal views of embryos at the 7- (A) or 12- (B,C) somite stages, showing the expression of wnt2ba (A,B) and ors2 (C), as revealed by mRNA in situ hybridization. (A) No expression of wnt2ba is visible at the 7-somite stage at the level of the intermediate mesoderm, whereas it is expressed at more anterior regions (inset). (B,C) The expression of wnt2ba is detected from the 12-somite stage (B, arrowhead) onwards in the kidney anlage, colocalizing with osr2 expression (C, arrowhead). (D-I) Transverse sections of 21-somite stage embryos with dorsal at the top. (D-F) Embryos showing the expression of osr1 (D), ors2 (E) or wnt2ba (F) genes in the kidney anlage. Inset in F shows a similar expression pattern for wnt2bb. (G-I). Embryos double stained for osr1 (G) or ors1 (H) and pax2a (Osr genes in purple and pax2a in red) and wnt2ba (purple) and osr2 (red) (I). The expression of all three genes overlaps in the pronephros (encircled). s, somite.