Fig. 3 The late ventricular region was marked by known myocardial markers nkx2.5 and vmhc, as well as mef2cb. (A) nkx2.5 transcript was expressed in the heart tube, the late ventricular region (arrowhead), and bilateral pharyngeal domain. The late ventricular region was marked by vmhc (B), but not by myl7 (C). mef2cb was expressed in the presumed bilateral heart progenitors at 17 hpf (E), in the heart cone (F), and heart tube (G). Expression became gradually localized to the posterior ventricle (D) and became down-regulated by 32 hpf (H). mef2cb was co-expressed with vmhc (compare I to J). Probing for mef2cb and myl7 expression at the same time showed that mef2cb is expressed outside the heart tube at 30 hpf (compare K to L).
Reprinted from Developmental Biology, 354(1), Lazic, S., and Scott, I.C., Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish, 123-133, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.