|
Fig. 5 Whole-mount in situ hybridization using no tail (ntl), tbx6 and tbx24 probes. A–L) Animal pole views of embryos at 6 hpf with dorsal to the right. A–D) ntl expression is normal in uninjected (A) (n = 36/36), eve/vent morphant (B) (n = 30/30), eve/vox morphant (C) (n = 22/22), and eve/ved morphant (D) (n = 43/43). E–H) tbx6 expression is normal in uninjected (E) (n = 20/20), eve/vent morphant (F) (n = 105/113), eve/vox morphant (G) (n = 77/93), and eve/ved morphant (H) (n = 75/76). I–L) tbx24 expression is normal in uninjected (I) (n = 27/27), eve/vent morphant (J) (n = 64/70), eve/vox morphant (K) (n = 66/68), but is expanded in eve/ved morphant (L) (n = 99/130). Black arrows indicate the normal bilateral tbx24 expression in MPCs. Black arrowhead indicates the expanded ventral expression of tbx24. Scale bar, 200 μm.
Reprinted from Developmental Biology, 349(1), Seebald, J.L., and Szeto, D.P., Zebrafish eve1 regulates the lateral and ventral fates of mesodermal progenitor cells at the onset of gastrulation, 78-89, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.