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Fig. 6 Neither iridoblast nor melanoblast markers co-localize with xanthoblast markers. (A–L) Confocal images collected from lateral aspect of anterior tail region of fixed zebrafish, 20x. (A–C,M) Wild-type embryo reveals that csf1r signal is not localized with pnp4a expression at 24 hpf (A) csf1r (B) pnp4a. (C) Color merged: green: csf1r mRNA, red: pnp4a mRNA (inset 40x). (D–F,N) Wild-type embryo reveals that aox3 signal is not localized with pnp4a expression at 24 hpf (D) aox3 (E) pnp4a. (F) Color merged: green: aox3 mRNA, red: pnp4a mRNA (inset 40x). (G–I) Wild-type embryo reveals that csf1r signal is not localized with dct expression at 24 hpf (G) csf1r (H) dct. (I) Color merged: green: csf1r mRNA, red: dct mRNA (inset 40x). (J–L) Wild-type embryo reveals that aox3 signal is not localized with dct expression at 24 hpf (J) aox3 (K) dct. (L) Color merged: green: aox3 mRNA, red: dct mRNA (inset 40x). (M,N) Percent of overlap between chromatoblast markers (see Tables 2 and 3). (M) Green line = % of csf1r+ cells that are pnp4a+/csf1r+. Red line = % of pnp4a+ cells that are pnp4a+/csf1r+. (N) Green line = % of aox3+ cells that are pnp4a+/aox3+. Red line = % of pnp4a+ cells that are pnp4a+/aox3+. Scale bars: (A–L) 60 μm; (C,F,I,L inset) 30 μm.
Reprinted from Developmental Biology, 344(1), Curran, K., Lister, J.A., Kunkel, G.R., Prendergast, A., Parichy, D.M., and Raible, D.W., Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest, 107-118, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.