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Fig. 4

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ZDB-IMAGE-100603-23
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Figures for Deacon et al., 2010
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Fig. 4 miR-143 is required and sufficient for CM growth and elongation. (A-I) At 48 hpf, WT, MO143-injected and miR-143-overexpressing zebrafish embryos carrying a cmlc2::GFP transgene (green) were immunostained with anti-DM-GRASP antibodies (red) and imaged by confocal microscopy. Shown are flattened z-stacks (A,D,G) and individual sections (B,C,E,F,H,I) from each group. Dashed lines demarcate the approximate outer curvature/inner curvature (OC/IC) boundary based on proximity to the atrioventricular junction. (J,K) The circularities (J) and areas (K) of cells in the IC and OC of WT, MO143 and miR-143-overexpressing (mimic) embryos. Asterisk indicates statistical significance compared with WT. (L,M) In situ hybridization for nppa expression in WT (L) and MO143 (M) animals. The perimeters of the ventricles are highlighted with dashed yellow lines and the OC (nppa expressing) and IC (nppa non-expressing) populations are indicated. (N-R) Chimeric ventricles were derived by transplantation of WT (N,Q,R) or MO143 (O,P,Q,R) blastula cells carrying the cmlc2::GFP transgene. (N-P) Mosaic hearts were immunostained with anti-DM-GRASP to outline cell borders. Representative images of WT (N) and MO143 cells (O,P, green, *) in the OC. A morphant cell is shown projected from the ventricular wall (P, arrowhead). (Q,R) The average circularity and area for WT and MO143 cells in the OC and IC.

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