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Fig. 1 Pancreatic expression patterns of nkx6.1 and nkx6.2. Left panel: Whole-mount in situ hybridizations (WISHs) showing expression of nkx6.1 (A,C,E, G) and nkx6.2 (B,D,F, H) at 4 somites (A,B), 18 somites (C,D), 24 hpf (E,F), and 48 hpf (G,H). Right panel: Double fluorescent WISHs showing expression of nkx6.1 and foxa1 (A–C) or neuroD (nrd) (G–I) and of nkx6.2 and foxa1 (D–F) or neuroD (J–L) at 6 somites. Z-plane confocal images. All views are lateral with anterior part to the left. Scale: 20 μm.
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Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.