ZFIN Image: Chablais et al., 2010, Fig. 5

Image

Figure Caption

Fig. 5 IGF signaling is required for the expression of blastemal and wound-epidermal marker genes. Whole-mount in situ hybridization of fins with antisense probes (purple). (A-D) In control fins at 36 hpa, fgf20a and msxB are strongly detected in the blastema of the outgrowth (A,C). In fins treated with NVP-AEW541, the expression of the blastemal marker genes is almost undetectable (B,D). (E-J). Drug-shift experiment (30 hours at normal conditions and 24 hours with treatment). Control fins express msxB in the blastema (E), wnt5b in the apical wound epidermis and distal tip of the blastema (G), and lef1 in the proximal wound epithelium and distal blastema (I). Exposure to NVP-AEW541 (F,H,J) strongly diminished expression of these genes. Scale bar: 100 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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