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Fig. 5 Effects of nestin MO injection on ascl1b (marker of NPC), Elavl3/4 (marker of mature neurons) and otx2 (marker of midbrain tissue).
A. Ascl1b expression was analyzed by WMISH at 25 hpf. Upper panel shows dorsal view with deletion of yolk. Lower panel shows lateral view, head to the left. B. Elavl3/4 was analyzed by confocal microscopy at 25 hpf. C. Otx2 was analyzed by WMISH at 25 hpf. Scale bar: 200 μm.
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