Fig. 3

Fig. 3 Effects of smad3b overexpression on the expression of early neural and non-neural markers. (A–L) Embryos were injected at the one-cell stage at indicated doses and examined by in situ hybridization for marker gene expression at around 60% epiboly stage. Embryos were positioned laterally with dorsal to the right and animal pole to the top. Note that smad3b overexpression enhanced ventral expression but inhibited dorsal expression of sox2 and sox3. In contrast, smad3b overexpression inhibited expression of gata2. (M–O) Bar graphs showing percentage of embryos with different expression levels of the markers. The number of observed embryos was indicated in the parentheses. For sox2 and sox3, the percentage (red) of embryos with ventral enhancement was calculated. For gata2, the percentage (yellow) of embryos with decreased expression was calculated regardless of decreasing extents. (P–R) Quantitative RT-PCR results of sox2 (P), sox3 (Q) and gata2 (R). See Fig. 1 legend for interpretation.