Fig. 1

Fig. 1 Expression of dzip1, sufu and hip in the otic region of 26hpf wild-type, ptc1^-/-; ptc2^-/- double mutants and smo^-/- single mutants. (A–H) Levels of dzip1 and sufu are unaltered in ptc1^-/-; ptc2^-/- and smo^-/- embryos, in which Hh signalling is severely up- and down-regulated, respectively. (I–L) hip expression is strongly up-regulated in ptc1^-/-; ptc2^-/- embryos and down-regulated in smo^-/- embryos. For comparison, each set of mutants and siblings (A–L) were stained in the same well and photographed using the same light intensity. (M and N) Hand cut transverse section (M) and lightly stained whole-mount (N) ptc1^-/-; ptc2^-/- specimens showing that, although highly up-regulated, hip expression remains regionalised in the ptc1^-/-; ptc2^-/- embryo. All panels, except M, are dorsal views of the hindbrain and otic region of whole-mount 26hpf embryos. Anterior is to the top except in panels K and L, where anterior is to the left, and panel M, which is a transverse hand cut section at the level of the otic vesicle, dorsal to top. Abbreviations: g, statoacoustic ganglion; n, notochord; nt, neural tube; o, otic vesicle.