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Fig. 1 Differential regulation of col11a2 expression by sox9a and sox9b. (A–D) Lateral view and (E–H) ventral view of 2 dpf embryos. Expression of col11a2 appeared in neurocranium (ethmoid plate, arrowhead), pharyngeal arches (mandibular and ceratohyal, arrows), otic vesicle and in the pectoral fin bud (A, E). Expression in neurocranium and pharyngeal arches was lost in the sox9a mutant (B, F). In the sox9b mutant, expression disappeared from the pharyngeal cartilages, but expression in the neurocranium (arrowhead) remained (C, G). Expression almost disappeared in sox9a;sox9b double mutant embryos (D, H). At 2dpf, sox9a was expressed in the neurocranium (arrowhead) and pharyngeal arches (arrows) in locations corresponding to col11a2 expression (I), but sox9b was only expressed in mandibular and ceratohyal (arrows), not in the ethmoid plate and trabeculae (J). These results confirmed microarray results showing that col11a2 is down-regulated in sox9 mutant embryos, and revealed that sox9a and sox9b regulate col11a2 expression in a tissue specific manner according to sox9 expression patterns. ch, ceratohyal; ep, ethmoid plate; ma, mandibular; ov, otic vesicle; op, opercle; pf, pectoral fin bud.
Reprinted from Developmental Biology, 329(1), Yokoi, H., Yan, Y.L., Miller, M.R., Bremiller, R.A., Catchen, J.M., Johnson, E.A., and Postlethwait, J.H., Expression profiling of zebrafish sox9 mutants reveals that Sox9 is required for retinal differentiation, 1-15, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.