ZFIN Image: Stedman et al., 2009, Fig. 1

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Figure Caption

Fig. 1 irx1b and irx7 are required for the expression of krox20 in r3. Embryos uninjected (ni; A, E, I, K, M) or injected with the morpholino against irx7 (Mo7; B,F) or against irx1b (Mo1b; C, G), coinjected with both morpholinos (Mo1bMo7) (D, H, J, L, N) or injected with Mo1b, Mosp7 and irx7 and gfp RNAs (O, P), were stained by ISH with probes indicated on the left and colour-coded. Panels A–D are lateral views of whole embryos, and panels E–P are dorsal views of flat-mounted embryos. Panel P is a high magnification of part of the hindbrain of a partially rescued morphant, which shows that krox20 expression in r3 is restored specifically in gfp and irx7 expressing cells (arrows). Rostral is toward the left. The embryonic stage is indicated at the bottom-right of each picture. Arrowheads in panels J, L, N indicate the absence of krox20 expression at the position of r3 in double Mo1bMo7 morphants.

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Acknowledgments

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Reprinted from Developmental Biology, 327(2), Stedman, A., Lecaudey, V., Havis, E., Anselme, I., Wassef, M., Gilardi-Hebenstreit, P., and Schneider-Maunoury, S., A functional interaction between Irx and Meis patterns the anterior hindbrain and activates krox20 expression in rhombomere 3, 566-577, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.