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Fig. 4 Cardiac cone formation occurs normally in nkx-deficient embryos. In situ hybridization depicts expression of cmlc2 (A,B), vmhc (C,D), and amhc (E,F) in wild-type and nkx-deficient embryos. Embryos viewed dorsally, anterior to the top, at the 22-somite stage. (A,C,E) Wild-type embryos exhibit normal fusion of the bilateral cardiac precursors, creating an intact cardiac cone. (B,D,F) nkx-deficient embryos mirror the expression patterns observed in wild-type, with the exception of a slightly enlarged and elongated lumen within the cardiac cone.
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Reprinted from Developmental Biology, 322(2), Targoff, K.L., Schell, T., and Yelon, D., Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number, 314-321, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.