ZFIN Image: Leung et al., 2008, Fig. 3

Image

Figure Caption

Fig. 3 gpr161 knockdown disrupts L–R identity in lateral plate mesoderm. (A) lefty2 expression in the left anterior lateral plate mesoderm (LPM) and lefty1 expression in the left diencephalon were disrupted in gpr161 knockdown embryos. (B) Graphical summary of control (99.3 ± 1.1%; n = 351), gpr161 morpholino (MO#24, 67.0 ± 4.7%; n = 348) and t-test (p < 0.01) results were from 11 injection experiments. (C) spaw expression in the left anterior lateral plate mesoderm (LPM) at 19.5 hpf in control and gpr161 knockdown embryos. ntl expression marked the midline as a reference. (D) Graphical summary of spaw expression in control (98.7 ± 1.4%; n = 311), gpr161 morpholino (MO#24, 63.4 ± 2.7%; n = 306) and t-test (p < 0.01) results were from 13 injection experiments. Error bars were ± SEM. All scale bars were 100 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.