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Fig. S4 Gross brain and eye patterning are unaffected in ast and slit1a morphant embryos. In situ hybridisation of 76 hpf WT, ast (ti272z) and slit1a morphant embryos with antisense mRNA probes for mbx-s (A-C), fgf8 (D-F), robo4 (G-I) and tbx5 (J-L). No changes in the expression patterns of these genes were observed between WT, ast (ti272z) or slit1a morphant embryos. Tec, tectum; MHB, midbrain-hindbrain boundary. Lateral views of wholemounts (A-I). Eyes only (J-L). Anterior left, dorsal up. Scale bar = 100μm. Supplemental
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