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Fig. 5 Whole-mount in situ hybridization of blood markers to MO-injected embryos at 18 hpf. Wild-type (no injection), Zebb302a, and Zebb302c injected fish were collected at 18 hpf. Fish were treated with antisense cRNA probes for gata-1 (erythroid development, A–C), gata-2 (early hematopoietic stem cell, D–F), and the myeloid marker pu1 (G–I). All embryos were photographed in 70% glycerol/PBS using a Nikon dissecting microscope.
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Reprinted from Developmental Biology, 283(1), Yergeau, D.A., Cornell, C.N., Parker, S.K., Zhou, Y., and Detrich, H.W. 3rd., bloodthirsty, an RBCC/TRIM gene required for erythropoiesis in zebrafish, 97-112, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.