Fig. 2
Fig. 2 SLC20A1 expression during zebrafish embryogenesis. Whole-mount in situ hybridization with the indicated DIG-labeled antisense probes at the indicated stages. (A,B,E,G–J) Lateral views with anterior towards the right. (C,D) Transversal sections of the embryo shown in B at the levels indicated. (F) Dorsal view. (K,L) Costaining for NBC1 or ClC-K and ret1. ClC-K posterior limit of expression extends further than that of NBC1 and appears to correspond to the anterior limit of ret1. (M–O). Double in situ hybridization with ClC-K (green) and PDZK1 (red) indicates that a cell population in the middle of the pronephros expresses both ClC-K and PDZK1 (yellow). Arrowheads show the distinct posterior limit of the expression of the indicated markers. Abbreviations: cv, caudal vein; pcv, posterior caudal vein; pnd, pronephric duct segment; s, somites.
Reprinted from Gene expression patterns : GEP, 6(7), Nichane, M., Van Campenhout, C., Pendeville, H., Voz, M.L., and Bellefroid, E.J., The Na(+)/PO(4) cotransporter SLC20A1 gene labels distinct restricted subdomains of the developing pronephros in Xenopus and zebrafish embryos, 667-672, Copyright (2006) with permission from Elsevier. Full text @ Gene Expr. Patterns