Fig. 2 RNA in situ hybridization analysis of 72 hpf zebrafish embyros. (A) Lack of whna expression in thymic rudiment; note expression in developing eye and brain structures. (B) Expression of whnb in third branchial arch; arches are indicated by Roman numbers. (C) Transverse section through thymic primordium indicating the location of whnb-expressing cells (purple). ov, otic vesicle; ph, pharynx; no, notochord; y, yolk (with non-specific staining); op, operculum. Only one half of the section is shown. (D–F) Expression of whnb in developmental mutants. D, wild-type embryo. E, cloche mutant; F, casanova mutant.
Reprinted from Mechanisms of Development, 118(1-2), Schorpp, M., Leicht, M., Nold, E., Hammerschmidt, M., Haas-Assenbaum, A., Wiest, W., and Boehm, T., A zebrafish orthologue (whnb) of the mouse nude gene is expressed in the epithelial compartment of the embryonic thymic rudiment, 179-185, Copyright (2002) with permission from Elsevier. Full text @ Mech. Dev.