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Fig. S3 gata4 and gata6 expression in embryos in which Fgf signaling was blocked at 22 and 26 hpf. Embryos obtained from outcrossing a hemizygous Tg(hsp70l:dnfgfr1-EGFP) zebrafish were heat shocked at 22 (A-C,G-H) or 26 (D-F,I-K) hpf, harvested at 35-36 hpf, and examined for gata4 (A-E) and gata6 (F-K) expression. The percentage of hemizygous embryos exhibiting a similar expression pattern is indicated in the lower left-hand corner (n=8-10). (A-K) The expression of gata4 and gata6 in the liver region of the hemizygous embryos heat shocked at 22 hpf was similar to that in the embryos heat shocked at 26 hpf and only weakly reduced compared with wild-type siblings. Arrows point to the liver region. All images are dorsal views, anterior left.