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Fig. 4 Zygotic nos1 is required for adult germline maintenance. Histology of 6-month-old females (A and B) and male (C and D) gonads. (A) Wild-type ovaries contain oocytes (stages indicated). (B) nos1 mutant females do not contain oocytes. The testes of wild-type (C) and nos1 mutants (D) are indistinguishable. Three-month-old wild-type (E, G and I) and nos1 mutant (F, H and J) ovaries freshly isolated (E and F) or stained for vasa (G and H) or ziwi (I and J) RNA. Wild-type ovaries contain numerous stage I oocytes, visualized as small (7–140 μm diameter) clear cells on the surface of the ovary (E) or as small vasa RNA+ (G) and ziwi RNA+ (I) cells (arrows). In contrast, nos1 mutant ovaries contain very few stage I oocytes (F, H, J; arrows in panels H and J). RNA in situ hybridization to detect vasa RNA reveals that 35 dpf nos1 mutant ovaries (L) contain fewer < 20 μm germ cells (arrow) than wild-type ovaries (K). sg, spermatogonia; sp, sperm; l, liver. Scale bars: 100 μm in panels A–D, K, L; 200 μm panels in E–J.
Reprinted from Developmental Biology, 305(2), Draper, B.W., McCallum, C.M., and Moens, C.B., nanos1 is required to maintain oocyte production in adult zebrafish, 589-598, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.