Fig. 6 Influence of the her12–ORF-Mo on gene expression in the PSM. (A, C, E) Wild type expression of her1, her7 and deltaC, respectively. (B, D, F) Expression of her1, her7 and deltaC, respectively, after her12–ORF-Mo injection (0.8 mM; 3 experiments for her1, n = 115, 55% affected; 2 experiments for her7, n = 65, 51% affected; 2 experiments for deltaC, n = 72, 42% affected). (G, H) Wild type expression of spt, (I, J) spt expression in her12 ORF-morphants (0.8 mM, 2 experiments, n = 68, 96% of all embryos show wild type expression). (A–F) Flat-mounted embryos (8- to 10-somite stage), anterior to the top. (G–J) Whole mount embryos (10-somite stage); (G, I) dorsal view, posterior downwards; (H, J) lateral view, anterior to the left.
Reprinted from Developmental Biology, 304(2), Shankaran, S.S., Sieger, D., Schroter, C., Czepe, C., Pauly, M.C., Laplante, M.A., Becker, T.S., Oates, A.C., and Gajewski, M., Completing the set of h/E(spl) cyclic genes in zebrafish: her12 and her15 reveal novel modes of expression and contribute to the segmentation clock, 615-632, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.