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Waxman et al., 2007 - Comparison of the expression patterns of newly identified zebrafish retinoic acid and retinoid X receptors

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Fig. 1 Sequence, phylogeny, and RT-PCR of zebrafish RARs. A: Sequence alignment of zebrafish RARs. Alignment was performed with ClustalW. B: Phylogenetic analysis of all available full-length sequences. Another nuclear receptor, human PPAR, was defined as the outgroup. Phylogeny was constructed using the neighbor-joining method with PAUP*. Numbers on the nodes reflect bootstraps from 1,000 replicates. C: RT-PCR for the zebrafish rar genes from cDNA collected from 25 2-8 cell or 15-20 somite embryos. -RT control indicated lack of genomic DNA contamination (not shown). Asterisk indicates the newly identified gene rargb.

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