Fig. 4

Fig. 4 Pigment cell progenitors show deficits in the mos^m188 mutants. Expression analysis of pigment specific genes in wild type (A, C, E, G) lateral and (A', C', E', G') dorsal views, and in mos^m188 mutants (B, D, F, H) lateral and (B', D', F', H') dorsal views as revealed by whole mount in situ hybridization in 22 hpf embryos. A-B': Expression of mitfa/nac, the early marker of pigment progenitors, is significantly downregulated in the trunk region while the mid-hindbrain boundary domain is unaffected in mos^m188 mutants. C-D': Similarly, the melanophores specific dct/trp2 expression is largely absent in mos^m188 mutants at this stage. E-F':Fms that specifies melanophore and xanthophore lineages is essentially absent in mos^m188mutants as is xanthine dehydrogenase (G-H';), the enzyme in pteridine synthesis pathway that marks lineage-committed xanthoblasts.