ZFIN Image: Raymond et al., 2006, Fig. 4

Image

Figure Caption

Fig. 4 Molecular profile of the injury-induced proliferating retinal progenitors is similar to retinal stem cells in the CMZ. A) At 4 dpl deltaC expression (dlc; magenta) is upregulated in the CMZ (arrow) and in cells in the INL beneath the lesion (asterisks). Scale bar = 150 μm. B) At 4 dpl, cells in the CMZ (arrow) and lesioned area labeled with a 2-hour pulse of BrdU (green) also express deltaC (magenta). C) notch1b (n1b; green) and D) notch3 (n3; green) are also up-regulated but are generally not co-expressed with deltaC (magenta). E) At 4 dpl, N-cadherin immunoreactivity (Cdh2; magenta) is strongly up-regulated in the lesioned area (asterisks) and diffusely localized. Inset: zrf1, anti-zebrafish-GFAP (green) in radial fibers of Müller glia in the lesioned retina (7 dpl) co-localizes with N-cadherin immunoreactivity (magenta). F) At 7 dpl, BrdU⁺nuclei (green) associate with Müller glial radial fibers that are strongly immunoreactive for N-cadherin (magenta). DAPI (blue). G) At 3 dpl, activated Müller glia confined to the lesioned region (asterisks) express BLBP (magenta), a marker of immature Müller glia, and they are mitotically active (PCNA⁺, green). H) Radial fibers of injury-activated Müller glia are zrf1⁺(blue). Proliferating Müller nuclei are PCNA⁺(green) and many have migrated to the apical surface (the former ONL where photoreceptors are missing) and are BLBP⁺(magenta).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.