ZFIN Image: Jin et al., 2006, Fig. 4

Image

Figure Caption

Fig. 4 The enhanced green fluorescence protein (EGFP) protein is colocalized with embryonic βe1-globin, pu.1, and flk1 transcripts in the Tg(5' 5kbscl:EGFP)^a transgenic fish. A,B: Lateral views of the 20-somite stage (18s) Tg(5' 5kbscl:EGFP)^a embryo stained with the anti-EGFP antibody (green) and antisense embryonic βe1-globin RNA probe (red) show the EGFP protein expression and the endogenous embryonic βe1-globin transcript. A',B<',A'/B': Confocal images (original magnification, x25) of the boxed region in A and B indicate that the EGFP protein (green) is colocalized with embryonic βe1-globin transcript (red). C,D: Lateral views of the 18-somite stage (18s) Tg(5' 5kbscl:EGFP)^a embryo stained with the anti-EGFP antibody (green) and antisense pu.1 RNA probe (red) show the EGFP protein and pu.1 RNA expression. C',D',C'/D': Confocal images (original magnification, x50) of the boxed region in C and D confirm that the EGFP protein (green) is colocalized with pu.1 (red). E,F: Lateral views of the 22 hours postfertilization (hpf) stage Tg(5' 5kbscl:EGFP)^a embryo stained with the anti-EGFP antibody (green) and antisense flk1 RNA probe (red) indicate the EGFP protein and flk1 RNA expression. E',F',E'/F': Confocal images (original magnification, x25) of the boxed region in E and F show the colocalization of the EGFP protein (green) and flk1 (red). In all the panels, embryos are oriented with anterior to the left.

Figure Data

Expression / Labeling details

Acknowledgments

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