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Fig. 4

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ZDB-IMAGE-051221-4
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Figures for Chen et al., 2005
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Fig. 4 def encodes a nuclear protein, and its expression is enriched in the endoderm organs. (A, top panel, lanes 2-7) RNA gel blot hybridization revealed that def had two transcribed forms that peaked at 12 hpf and 1 dpf and then gradually reduced to a lower level at 5 dpf. (Lane 9) The shorter form was more abundant in the adult liver (AL). (Lane 8) def expression is undetectable in the defhi429 mutant. (elf1a) elongation factor 1a. (B) Immunostaining using anti-Def antibody showed that Def is a nuclear-localized protein. (C-F) Whole-mount in situ hybridization using a def riboprobe to examine the expression patterns of def at 2 dpf (C), 3 dpf (D), 4 dpf (E), and 5 dpf (F). def expression was specifically enriched in the digestive organs from 3 to 5 dpf. (in) Intestine; (lv) liver; (p) pancreas; (sw) swimbladder. (G,H) Sectioning in situ hybridization showed that def expression is specifically enriched in the intestine (in), liver (lv), and exocrine pancreas (ex) but is excluded from the endocrine pancreas (en) at 3 and 4 dpf.

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