FIGURE 1

Fat2 protein structure and anatomy of the zebrafish cerebellum. (a) Schematic representation of the domain organization of wild‐type and mutant Fat2 proteins. The wild‐type Fat2 protein consists of 4342 amino acids. The fat2^nub128 allele carries a frameshift mutation and encodes a 46‐amino acid peptide, of which 43 amino acids are derived from the Fat2 and the remaining 3 are unrelated. In contrast, the fat2^vu381 allele carries a nonsense mutation and encodes a truncated protein of 866 amino acids. (b) Schematic illustration of the anatomy of the zebrafish larval cerebellum and its neural circuits, showing axonal trajectories of granule cells in the cerebellar corpus (CCe) (i, iv), granule cells in the eminentia granularis (EG) (ii, v), and granule cells in the lobus caudalis (LCa) (iii, vi), together with a dorsal view of the cerebellum. Green dots indicate granule cells (GCs), magenta dots indicate Purkinje cells (PCs), and blue dots indicate crest cells (Crs). Parallel fibers (PFs) are shown in panel (iv).