FIGURE

Fig. 3

ID

ZDB-FIG-260515-35

Publication

Lim et al., 2026 - Functional impact of the ATP1A3-p.A813V variant: insights into a calcium-driven hyperexcitability cascade in rapid-onset dystonia-Parkinsonism

Other Figures

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Fig. 3

ATP1A3 loss-of-function elicits neuronal hyperexcitability. (a–d) expression of c-fos messenger RNA (a marker of neuronal activity) at 2 days post-fertilization (DPF) in atp1a3b^+/+ (a, c) and atp1a3b^-/- (b, d) zebrafish. Dorsal views of the brain (a, b) and lateral views of the spinal cord (c, d) are presented. Scale bar: 250 μm (a, b), 500 μm (c, d). (e, f) representative transverse section images of the spinal cord in 2 DPF atp1a3b^+/+ (e) and atp1a3b^-/- (f) zebrafish. Immunohistochemistry labeling with anti-Hu antibody (green) and anti-pERK (red) highlights neuronal populations and phosphorylated ERK. Scale bar: 10 μm. (g) Quantification of pERK+ Hu+ neurons per section. Unpaired t-test; n = 8

Expression Data

Gene:	fosab
Antibodies:	Ab1-elavl Ab20-mapk
Fish:	atp1a3b^km29/km29
Anatomical Terms:	brain neuron spinal cord neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp1a3b^km29/km29
Observed In:	brain neuron spinal cord neuron
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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