FIGURE

Fig. 4

ID

ZDB-FIG-260506-56

Publication

Zheng et al., 2026 - BMP-Smad1/9 signaling plays a critical role in regulating zebrafish PGC proliferation

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Fig. 4

PGC-specific knockout of <italic toggle='yes'>smad9</italic> leads to reduced PGC numbers.

a Representative ddx4 ISH images of sibling and smad9-cKO embryos. The gonad region was enlarged for better visibility. b Effect of smad9-cKO on PGC number. Left, representative images showing PGC development in 3 dpf sibling and smad9-cKO embryos. Right, box plot showing PGC numbers per embryo. The median is indicated by the center line. The bottom, top edges, and whiskers represent the min and max values and 1.5 times the IQR, respectively. c Quantification of PGC numbers in different mutants. Left, box plot showing PGC numbers per embryo in sibling, smad1-cKO, smad9-cKO, and smad1&9-cKO embryos at 2 dpf. The median is indicated by the center line. The bottom, top edges, and whiskers represent the min and max values and 1.5 times the IQR, respectively. Each dot represented one embryo. Right, representative images of PGCs in the corresponding embryos. PGCs were labeled by kop:Eos. Dotted circles marked cryaa:CFP in the lens of smad9-cKO embryos; red fluorescence indicated gsc:tdTomato in smad1-cKO embryos. NT, notochord. White arrowheads indicated cellular debris. Statistical analysis was performed using a two-sided Student’s t-test. Ne, number of embryos analyzed. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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