FIGURE

Fig. 5

ID

ZDB-FIG-260506-57

Publication

Zheng et al., 2026 - BMP-Smad1/9 signaling plays a critical role in regulating zebrafish PGC proliferation

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Fig. 5

<italic toggle='yes'>smad1</italic>-cKO PGCs exhibit defective proliferation and increased apoptosis.

a Representative tracking images of dividing PGCs in sibling and smad1-cKO embryos from germ ring to 90% epiboly stage. The kop:Eos-labeled PGCs were marked by red and white asterisks. b Ratio of dividing PGCs in embryos shown in (a). c Detection of proliferating PGCs via EdU incorporation assay. Embryos were treated with EdU from the shield to the bud stage and collected at the 2-somite stage for immunostaining with EdU and Ddx4. Top panel, schematic of assay procedure; lower left, representative images of PGCs (red). Ddx4- and EdU-positive PGCs were indicated by yellow arrowheads, and Ddx4-positive but EdU-negative PGCs by white arrows. Lower right, bar graph showing the percentage of EdU-positive and EdU-negative PGCs in sibling and smad1-cKO embryos. d Live images of smad1-cKO PGCs undergoing apoptosis. Top panel, piwil1:mCherry-labeled PGCs in the gonad region; bottom panel, time-lapse images showing PGCs undergoing apoptosis. Yellow arrowheads marked surviving PGCs, white arrows indicated apoptotic PGCs, and asterisks denoted apoptotic cell debris. e Immunostaining of active Caspase-3 (green) and mCherry (PGCs, red) in sibling and smad1-cKO embryos at 2 dpf. Left panel, representative images of PGCs in the gonad region. Active Caspase-3–positive and –negative PGCs were indicated by white arrows and yellow arrowheads, respectively. Right, bar graph showing the percentage of active Caspase-3–positive or –negative PGCs. Statistical significance was determined using a two-sided Fisher’s exact test. Nc, number of observed PGCs. Source data are provided as a Source Data file.

Expression Data

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Antibody Labeling

Phenotype Data

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