Biliary-mediated liver regeneration is inhibited in the pdgfra mutant. (A) Experimental scheme illustrating the treatment of Mtz or DMSO and analysis at 6 dpf, 24 h after injury (at regeneration 24 h; R24 h) and R48 h. (B) Confocal projection images showing the liver development and regeneration in wild type and mild pdgfra mutant at 6 dpf, R24 h and R48 h. Scale bars: 50 μm. (C) Whole-mount in situ hybridization images showing the expression of gc, cp and ttr in wild-type and mild pdgfra mutant livers (arrows) at R48 h. (D) Single-optical section images showing Bhmt and Dendra2 expression in wild type and mild pdgfra mutant at R48 h. Scale bars: 50 μm. (E) Statistical analysis of the percentage of Bhmt+ cells among Dendra2+ cells. n=6 (wild type) and n=5 (mild pdgfra−/−). (F) Single-optical section images showing Alcam and Dendra2 expression in wild type and mild pdgfra mutant at R48 h. Arrowheads indicate Dendra2+/Alcam− cells; arrows indicate Dendra2+/Alcam+ cells. Scale bars: 50 μm. (G) Statistical analysis of the percentage of Alcam+ cells among Dendra2+ cells. n=6. Numbers in B and C indicate the proportion of larvae exhibiting the expression shown. ****P<0.0001 (unpaired t-test). Data are mean±s.e.m.
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