|
Vgll4b suppresses Yap1-Tead activity more efficiently than Vgll4l in the pLLP. a–f” Single planes confocal images showing the expression of GTIIC:d2EGFP in the pLLP of 20 hpf embryos, uninjected (a) or injected with tBFP-yap1 (b), tRFP-vgll4b (c), vgll4l (d), tBFP-yap1 and tRFP-vgll4b (e), tBFP-yap1 and vgll4l (f) mRNA. The prim-mCherry transgene is used to visualize the pLLP (a’–f’). Merged images of the two channels are shown in (a”–f”). Quantifications of the mean intensity of GTIIC:d2EGFP measured on the average Z-stack projection of the whole image (g) or on a single plane within the pLLP in the same embryos (h) (N ≥ 1 independent replicates). Data are presented as mean ± SD. Unpaired t-tests (Mann–Whitney) were conducted. Scale bar: 20 μm (a–f”).
|
