Fig. 5

Vgll4 limits cell number in the pLLP through Yap1 Competition and Tead-Mediated Repression.

MIP of confocal Z-stacks showing the pLLP in vgll4b^+/+;vgll4l^+/+ (a), vgll4b^−/−;vgll4l^+/+ (c), vgll4b^+/+;vgll4l^−/− (e), vgll4b^−/−;vgll4l^−/− (g) embryos and the same genotypes, respectively, injected with tBFP-yap1 mRNA (b, d, f, h). i Quantification of pLLP cell number in each condition (N ≥ 2 independent replicates). MIP of confocal Z-stacks showing the pLLP in vgll4b^+/+;vgll4l^+/+;yap1^+/+ (j), vgll4b^+/+;vgll4l^+/+;yap1^−/− (k), vgll4b^−/−;vgll4l^+/+;yap1^+/+ (l), vgll4b^+/+;vgll4l^−/−;yap1^+/+ (m); vgll4b^−/−;vgll4l^−/−;yap1^+/+ (n), vgll4b^−/−;vgll4l^+/+;yap1^−/− (o), vgll4b^+/+;vgll4l^−/−;yap1^−/− (p), vgll4b^−/−;vgll4l^−/−;yap1^−/− (q) embryos. r Quantification of pLLP cell number in each condition (N ≥ 3 independent replicates, except for vgll4b^−/−;vgll4l^−/−;yap1^+/+, N = 1). MIP of confocal Z-stacks showing overview images of 32 hpf yap1^+/+ (s), yap1^−/− (t, t’), and embryos of the same genotypes injected with tBFP-vgll4b mRNA (u–v’), tBFP-vgll4bΔTDU2 mRNA (w–x’), or with vgll4l mRNA (y–z’). aa Percentage of pLLP displaying normal migration or “pLLP diving” in each condition (N ≥ 3 independent replicates, except for yap1^+/++ vgll4b, N = 2). Data are presented as mean ± SD. Unpaired t-tests (Mann–Whitney) were conducted. Scale bars: 20 μm (a–h, j–q) and 400 μm (s–z’).