FIGURE

Figure 1

ID

ZDB-FIG-260416-50

Publication

Anderson et al., 2026 - Tissue-Specific Expression of the EWSR1::FLI1 Fusion Protein Identifies col2a1a-Positive Cells as a Source of Ewing Sarcoma-like Tumors in Zebrafish

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Figure 1

Generation and validation of transgenic EWSR1::FLI1 zebrafish. (A) Schematic of ES translocation t(11;22)(q24;12) in EWSR1::FLI1 fusion showing the N-terminal domain of EWSR1, which contains transactivation domain (TAD), and its fusing with the C-terminal DNA-binding domain of the ETS transcription factor FLI1. (B) The col2a1a promoter is used to drive expression of the human EWSR1::FLI1 fusion construct in transgenic zebrafish created using the Tol2 transposase method. (C,D) Identification of transgene-positive larvae by GFP expression in the lens driven by the cryaa:EGFP cassette at 2 days post-fertilization (dpf). (E) Schematic showing location of human-specific primer set used to amplify fusion construct from RNA using semi-quantitative RT-PCR. (F) Detection of the 1502 bp EWSR1::FLI1 fusion transcript in transgene-positive larvae and ES cell line A673. (G) β-actin was used as the internal control for qRT-PCR. Gr+, green eye positive. Gr−, green eye negative.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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