Figure 1

Morphological assessment of zebrafish larvae during the experimental period. (A) Cumulative mortality recorded throughout the exposure period. Data are presented as mean ± SD from five independent replicates. No statistically significant differences were observed between treatment groups. (B) Larval body length measurements. Values represent mean ± SD from five independent replicates (10 larvae per treatment per replicate, n = 1). Different letters indicate statistical differences among treatments (p < 0.05). (C) Representative images illustrating the morphological classes assigned based on treatment-induced alterations. Scale bar = 500 μm. sb: swimbladder; y: yolk sac; h: head; e: eye; p: pericardium; t: tail. (D) Distribution of larvae across morphological classes at 72, 96, and 120 hpf for each treatment group. Data are expressed as mean percentages (n = 5, with 10 larvae evaluated per replicate). CTRL (E3 medium), ACE (0.05% acetone), 6-OHDA (250 µM), 6-OHDA+E5 (250 µM 6-OHDA + 5 µg/mL extract), 6-OHDA+E25 (250 µM 6-OHDA + 25 µg/mL extract), and E25 (25 µg/mL extract).