Figure 2

Tyrosine hydroxylase (TH) immunofluorescence at 120 hpf. (A) Representative whole-mount images of TH immunostaining in control, 6-OHDA, co-treatment, and extract-alone groups. Images were acquired under identical magnification, exposure time, illumination intensity, and gain settings. Scale bar = 500 µm. (B) Quantification of TH immunofluorescence intensity within anatomically defined regions of interest, expressed as fold of control. Data are presented as mean ± SD from five independent replicates (10 larvae per treatment per replicate, n = 1). Statistical comparisons were performed using the one-way ANOVA test followed by Tukey’s post hoc test. Different letters denote statistically significant differences among treatments (p < 0.05). CTRL (E3 medium), ACE (0.05% acetone), 6-OHDA (250 µM), 6-OHDA+E5 (250 µM 6-OHDA + 5 µg/mL extract), 6-OHDA+E25 (250 µM 6-OHDA + 25 µg/mL extract), and E25 (25 µg/mL extract).