Fig. 3

Activity and sleep in PARK7^-/- larvae are altered compared to wild types. Park7^-/- and wild type zebrafish larvae were exposed to MPP⁺ or vehicle from 3–5 dpf and thereafter washed and moved into LAM tubes for continuous behaviour analysis under light–dark cycles from 5–8 dpf. (A) Representative behaviour tracing of activity per 60 min. Y-axis shows number of beam crossings + /- SEM (n = 16). (B–G) Quantification of activity and sleep parameters from three individual experiments. Values are the mean ± SEM (n = 48) from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, different GLMs were created depending on the data distribution and scale followed by Tukey-adjusted comparisons of the model-adjusted means to assess the effects of genotype, drug and their interaction. (B, C) Activity in light (B) dark (C) phases. (D–G) Sleep analysis of LAM data: sleep bout duration (D); sleep latency (E); sleep ratio in light phases (F); sleep ratio in dark phases (G).